pNIC28-Bsa4
(Plasmid #26103)

• Purpose: (Empty Backbone) SGC Empty backbone for bacterial expression
• Depositing Lab: Opher Gileadi
• Publication: Savitsky et al J Struct Biol. 2010 Jun 10. ():.

Backbone

• Vector backbone: pET28a
• Backbone manufacturer: Novagen
• Backbone size (bp): 5353
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: None
• GenBank ID: EF198106
• Tag / Fusion Protein:
  • His - TEV (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Ligation-Independent cloning (destroyed during cloning)
• 3′ cloning site: Ligation-Independent cloning (destroyed during cloning)
• 5′ sequencing primer: T7F
• 3′ sequencing primer: T7R

Resource Information

• Articles Citing this Plasmid:
  • 32 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Primers for LIC cloning:

Upstream: add TACTTCCAATCCATG to the 5’ end (ATG in-frame with the desired coding
sequence).

Downstream: add TATCCACCTTTACTG to 5’ end of downstream primer; add termination
codon, if necessary.

Detailed cloning method available in the paper (Savitsky et al., J Struct Biol., 2010)