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Addgene

HA-HIF2α P531A-pBabe-puro
(Plasmid #26091)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26091 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBabe-puro
  • Backbone size w/o insert (bp) 5140
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Hypoxia inducible factor 2 alpha
  • Alt name
    HIF2a
  • Alt name
    EPAS1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2655
  • Mutation
    cDNA with point mutation P531A
  • GenBank ID
    HSU81984
  • Entrez Gene
    EPAS1 (a.k.a. ECYT4, HIF2A, HLF, MOP2, PASD2, bHLHe73)
  • Tag / Fusion Protein
    • HA-tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH I (not destroyed)
  • 3′ cloning site EcoR I (destroyed during cloning)
  • 5′ sequencing primer pBabe-5'
  • 3′ sequencing primer pBabe-3'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HA-HIF2α P531A-pBabe-puro was a gift from William Kaelin (Addgene plasmid # 26091 ; http://n2t.net/addgene:26091 ; RRID:Addgene_26091)

  • For your References section:

    Inhibition of HIF is necessary for tumor suppression by the von Hippel-Lindau protein. Kondo K, Klco J, Nakamura E, Lechpammer M, Kaelin WG. Cancer Cell. 2002 Apr . 1(3):237-46. 10.1016/S1535-6108(02)00043-0 PubMed 12086860
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