pBabe-puro-mIR-31
(Plasmid #26088)

• Depositing Lab: Bob Weinberg
• Publication: Valastyan et al Cell. 2009 Jun 12. 137(6):1032-46.

Backbone

• Vector backbone: pBABE-puro
• Backbone manufacturer: Weinberg lab (Addgene#:1764)
• Backbone size w/o insert (bp): 5169
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: miR-31
• Alt name: miR31
• Species: H. sapiens (human)
• Insert Size (bp): 400
• Entrez Gene: MIR31 (a.k.a. MIRN31, hsa-mir-31, miR-31)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: SalI (unknown if destroyed)
• 5′ sequencing primer: pBabe-5
• 3′ sequencing primer: pBabe-3

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The human miR-31 gene was PCR-amplified from genomic DNA and cloned into
pcDNA3.1 (Invitrogen), then subcloned into the BamHI and SalI sites of the pBABE-puro
retroviral vector.