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Addgene

pCA-ZtTA
(Plasmid #26083)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26083 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCA-HZ2
  • Backbone size w/o insert (bp) 5100
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    a loxP-flanked β-geo preceding the tetracycline transactivator tTA
  • Alt name
    ZtTA
  • Species
    E. coli
  • Insert Size (bp)
    6009

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (destroyed during cloning)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCA-ZtTA was a gift from Liqun Luo (Addgene plasmid # 26083 ; http://n2t.net/addgene:26083 ; RRID:Addgene_26083)

  • For your References section:

    Visualizing the distribution of synapses from individual neurons in the mouse brain. Li L, Tasic B, Micheva KD, Ivanov VM, Spletter ML, Smith SJ, Luo L. PLoS One. 2010 . 5(7):e11503. 10.1371/journal.pone.0011503 PubMed 20634890
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