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Addgene

pROSA26-ZtTA
(Plasmid #26082)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26082 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pROSA26-PA targeting vector
  • Backbone size w/o insert (bp) 12000
  • Vector type
    Mouse Targeting
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    a loxP-flanked β-geo preceding the tetracycline transactivator tTA
  • Alt name
    ZtTA
  • Species
    E. coli
  • Insert Size (bp)
    5100

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Pac I (not destroyed)
  • 3′ cloning site AscI (not destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

6bp deletion in Addgene sequence compared to author sequence. The discrepancy is in the vector before the BGH terminator and is not likely to affect function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pROSA26-ZtTA was a gift from Liqun Luo (Addgene plasmid # 26082 ; http://n2t.net/addgene:26082 ; RRID:Addgene_26082)

  • For your References section:

    Visualizing the distribution of synapses from individual neurons in the mouse brain. Li L, Tasic B, Micheva KD, Ivanov VM, Spletter ML, Smith SJ, Luo L. PLoS One. 2010 . 5(7):e11503. 10.1371/journal.pone.0011503 PubMed 20634890
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