pBTBXh-4
(Plasmid #26080)

• Purpose: (Empty Backbone)
• Depositing Lab: Ryan Gill
• Publication: Prior et al Biotechnol Bioeng. 2010 Jun 1. 106(2):326-32.

Backbone

• Vector backbone: pBTBXh-4
• Backbone size (bp): 4224
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Tetracycline, 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: None
• Tag / Fusion Protein:
  • His (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (unknown if destroyed)
• 3′ cloning site: HincII (unknown if destroyed)
• 5′ sequencing primer: atacccgttt ttttgggcta

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

There is an extra G in the middle of the alignment between Addgene's quality control sequence and the author's sequence. This discrepancy is in a non-coding region and does affect function.