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Addgene

pBTBX-4
(Plasmid #26070)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26070 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBTBX-4
  • Backbone size (bp) 4209
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Tetracycline, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    None

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (unknown if destroyed)
  • 3′ cloning site HincII (unknown if destroyed)
  • 5′ sequencing primer atacccgttt ttttgggcta
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene identified an additional Thr on the C-terminal of the Rep protein compared to the author's sequence. This discrepancy was found in similar plasmids from this deposit. There is also a 1 bp DNA mutation (C2861T) in the tonB terminator. It is unknown whether these cause functional differences.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBTBX-4 was a gift from Ryan Gill (Addgene plasmid # 26070)

  • For your References section:

    Broad-host-range vectors for protein expression across gram negative hosts. Prior JE, Lynch MD, Gill RT. Biotechnol Bioeng. 2010 Jun 1. 106(2):326-32. 10.1002/bit.22695 PubMed 20148414
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