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Addgene

pCI-Neo-His-Pitaire-Myc
(Plasmid #26066)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26066 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCI-neo
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 5472
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Pitaire
  • Alt name
    CrkRS
  • Species
    H. sapiens (human)
  • Entrez Gene
    CDK12 (a.k.a. CRK7, CRKR, CRKRS)
  • Tags / Fusion Proteins
    • His (N terminal on insert)
    • Myc (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer EBV-rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCI-Neo-His-Pitaire-Myc was a gift from Jonathon Pines (Addgene plasmid # 26066 ; http://n2t.net/addgene:26066 ; RRID:Addgene_26066)

  • For your References section:

    CrkRS: a novel conserved Cdc2-related protein kinase that colocalises with SC35 speckles. Ko TK, Kelly E, Pines J. J Cell Sci. 2001 Jul . 114(Pt 14):2591-603. PubMed 11683387
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