inactive cdk1 FRET sensor (2328)
(Plasmid #26065)

• Depositing Lab: Jonathon Pines
• Publication: Gavet et al Dev Cell. 2010 Apr 20. 18(4):533-43.

Backbone

• Vector backbone: pEYFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Cdk1 FRET Sensor- Inactive
• Mutation: Ser 126 mutated to Ala
• Tags / Fusion Proteins:
  • YFP variant (N terminal on insert)
  • CFP variant (C terminal on insert)
  • FLAG (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: CMV-F

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This is the Inactive form of the sensor, therefore Ser 126 is mutated to Alanine.

Original plasmid: pEYFP-C1 from Clontech, CMV promoter has been modified to introduce a T7 sequence and contains the extra sites HindIII, SacI and NcoI.

Domains: YFP variant-PoloBox Domain-flexible linker-Phospho site-CFP variant-FLAG