p-cyclinB1-mcherry (1958)
(Plasmid #26063)

• Depositing Lab: Jonathon Pines
• Publication: Gavet et al Dev Cell. 2010 Apr 20. 18(4):533-43.

Backbone

• Vector backbone: pmCherry-N1
• Backbone manufacturer: See note below
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Cyclin B1
• Species: H. sapiens (human)
• Entrez Gene: CCNB1 (a.k.a. CCNB)
• Tag / Fusion Protein:
  • mcherry (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: mCherry-R

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The backbone was derived from pECFP-N1. ECFP was replaced with mCherry.

There is a V5A mutation in cycB1. The mutation does not affect localization of the protein or it's degradation.