pCMX/cyclinB1 (17)
(Plasmid #26060)

• Depositing Lab: Jonathon Pines
• Publication: Hagting et al Curr Biol. 1999 Jul 1. 9(13):680-9.

Backbone

• Vector backbone: pCMX
• Backbone size w/o insert (bp): 4500
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Cyclin B1
• Species: H. sapiens (human)
• Entrez Gene: CCNB1 (a.k.a. CCNB)
• Tag / Fusion Protein:
  • Myc (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (unknown if destroyed)
• 3′ cloning site: BamHI (unknown if destroyed)
• 5′ sequencing primer: CMV-F

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

There is a V5A mutation in cycB1. The mutation does not affect localization of the protein or it's degradation.

For a wild type source of CyclinB1, please see plasmid 26062.