Venus-Cdc20 R132A (1768)
(Plasmid
#26059)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 26059 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepVenus-C1
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Backbone manufacturerSee note below
- Backbone size w/o insert (bp) 4700
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCdc20
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SpeciesH. sapiens (human)
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MutationMutation in the Mad2 binding site, R132A
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Entrez GeneCDC20 (a.k.a. CDC20A, OOMD14, OZEMA14, bA276H19.3, p55CDC)
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Tag
/ Fusion Protein
- Venus (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (unknown if destroyed)
- 3′ cloning site BamHI (unknown if destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
EYFP was swapped with Venus using AgeI and BsrGI restriction sites.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Venus-Cdc20 R132A (1768) was a gift from Jonathon Pines (Addgene plasmid # 26059 ; http://n2t.net/addgene:26059 ; RRID:Addgene_26059) -
For your References section:
The APC/C maintains the spindle assembly checkpoint by targeting Cdc20 for destruction. Nilsson J, Yekezare M, Minshull J, Pines J. Nat Cell Biol. 2008 Dec . 10(12):1411-20. 10.1038/ncb1799 PubMed 18997788