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pVenus-C1-Cdc20 (1534)
(Plasmid #26058)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26058 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pVenus-C1
  • Backbone manufacturer
    See note below
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cdc20
  • Species
    H. sapiens (human)
  • Entrez Gene
    Cdc20 (a.k.a. 2310042N09Rik, C87100, p55CD, p55CDC)
  • Tag / Fusion Protein
    • Venus (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

EYFP was swapped with Venus using AgeI and BsrGI restriction sites.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pVenus-C1-Cdc20 (1534) was a gift from Jonathon Pines (Addgene plasmid # 26058 ; http://n2t.net/addgene:26058 ; RRID:Addgene_26058)

  • For your References section:

    The APC/C maintains the spindle assembly checkpoint by targeting Cdc20 for destruction. Nilsson J, Yekezare M, Minshull J, Pines J. Nat Cell Biol. 2008 Dec . 10(12):1411-20. 10.1038/ncb1799 PubMed 18997788
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