ChR2-YFP-NavII-III
(Plasmid #26057)

• Depositing Lab: Matthew Grubb
• Publication: Grubb et al PLoS One. 2010 . 5(10):e13761.

Backbone

• Vector backbone: pLenti-Synapsin-hChR2(H134R)-EYFP-WPRE
• Backbone manufacturer: Addgene plasmid 20945
• Backbone size w/o insert (bp): 10093
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NavII-III
• Alt name: sodium channel subunit Nav1.2
• Alt name: sodium channel, voltage-gated
• Species: R. norvegicus (rat)
• Insert Size (bp): 684
• Tag / Fusion Protein:
  • EYFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BsrGI (unknown if destroyed)
• 3′ cloning site: BsrGI (unknown if destroyed)
• 5′ sequencing primer: CMV-F, EGFP-C
• 3′ sequencing primer: BGH-rev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The backbone was constructed by Karl Deisseroth at Stanford and is available at Addgene.
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry