YFP-NavII-III
(Plasmid #26056)

• Depositing Lab: Matthew Grubb
• Publication: Grubb et al Nature. 2010 Jun 24. 465(7301):1070-4.

Backbone

• Vector backbone: pEYFP-N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Nav1.2
• Alt name: sodium channel subunit Nav1.2
• Alt name: sodium channel, voltage-gated
• Species: R. norvegicus (rat)
• Insert Size (bp): 684
• Mutation: The insert codes only for the intracellular loop between transmembrane domains II and III
• Entrez Gene: Scn2a (a.k.a. NachII, Nav1.2, RII/RIIA, RNSCPIIR, SCN, Scn2a1, Scn2a2, ScpII)
• Tag / Fusion Protein:
  • YFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BsrGI (not destroyed)
• 3′ cloning site: BsrGI (not destroyed)
• 5′ sequencing primer: CMV-F

Resource Information

• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry