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Addgene

YFP-NavII-III
(Plasmid #26056)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26056 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pEYFP-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Nav1.2
  • Alt name
    sodium channel subunit Nav1.2
  • Alt name
    sodium channel, voltage-gated
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    684
  • Mutation
    The insert codes only for the intracellular loop between transmembrane domains II and III
  • Entrez Gene
    Scn2a (a.k.a. NachII, Nav1.2, RII/RIIA, RNSCPIIR, SCN, Scn2a1, Scn2a2, ScpII)
  • Tag / Fusion Protein
    • YFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsrGI (not destroyed)
  • 3′ cloning site BsrGI (not destroyed)
  • 5′ sequencing primer CMV-F
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    YFP-NavII-III was a gift from Matthew Grubb (Addgene plasmid # 26056 ; http://n2t.net/addgene:26056 ; RRID:Addgene_26056)
  • For your References section:

    Activity-dependent relocation of the axon initial segment fine-tunes neuronal excitability. Grubb MS, Burrone J. Nature. 2010 Jun 24. 465(7301):1070-4. 10.1038/nature09160 PubMed 20543823