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Addgene

mouse Drp1(K38A)
(Plasmid #26049)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26049 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1(-)Myc/His
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Growth instructions
    DH10B cells
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Drp1
  • Species
    M. musculus (mouse)
  • Mutation
    K38A GTPase mutant
  • Entrez Gene
    Dnm1l (a.k.a. 6330417M19Rik, Dlp1, Dnmlp1, Drp1, python)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Though this vector backbone has Myc and His tags, they are not in frame with the Drp1. Do not use these tags for purification or detection after expression.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mouse Drp1(K38A) was a gift from David Chan (Addgene plasmid # 26049 ; http://n2t.net/addgene:26049 ; RRID:Addgene_26049)

  • For your References section:

    Mitofusins Mfn1 and Mfn2 coordinately regulate mitochondrial fusion and are essential for embryonic development. Chen H, Detmer SA, Ewald AJ, Griffin EE, Fraser SE, Chan DC. J Cell Biol. 2003 Jan 20. 160(2):189-200. 10.1083/jcb.200211046 PubMed 12527753
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