pOPINE
(Plasmid #26043)

• Depositing Lab: Ray Owens
• Publication: Berrow et al Nucleic Acids Res. 2007 . 35(6):e45.

Backbone

• Vector backbone: pTriEx-2
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5457
• Vector type: Mammalian Expression, Bacterial Expression, Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: His-lacZ-KHHHHHH
• Tags / Fusion Proteins:
  • MAHHHHHHSSG (N terminal on insert)
  • KHHHHHH (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (unknown if destroyed)
• 3′ cloning site: MscI (unknown if destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pTriEx obtained from Merck
• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Promoters/baculoviral recombination sites: T7lacO, CMV enhancer and beta-actin promoter, p10 promoter/lef-2 and 1629 baculo elements.