pOPINVH
(Plasmid
#26041)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 26041 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepTriEx-2
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5457
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Vector typeMammalian Expression, Bacterial Expression, Insect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameu-LacZ-KHHHHHH
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Tags
/ Fusion Proteins
- u-phosphatase secretion leader (N terminal on insert)
- KHHHHHH (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (unknown if destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made bypTriEx obtained from Merck
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pOPINVH was a gift from Ray Owens (Addgene plasmid # 26041 ; http://n2t.net/addgene:26041 ; RRID:Addgene_26041) -
For your References section:
A pipeline for the production of antibody fragments for structural studies using transient expression in HEK 293T cells. Nettleship JE, Ren J, Rahman N, Berrow NS, Hatherley D, Barclay AN, Owens RJ. Protein Expr Purif. 2008 Nov . 62(1):83-9. 10.1016/j.pep.2008.06.017 PubMed 18662785
Map uploaded by the depositor.
