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Addgene

808 p3EmirCherry
(Plasmid #26034)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26034 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p3E
  • Backbone size w/o insert (bp) 2946
  • Vector type
    Entry vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mCherry
  • Insert Size (bp)
    758

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site attR2 (not destroyed)
  • 3′ cloning site attL3 (not destroyed)
  • 5′ sequencing primer M13pUC-fwd
  • 3′ sequencing primer M13rev
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

"p3E" refers to 3' entry clones flanked by R2-L3.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    808 p3EmirCherry was a gift from Nathan Lawson (Addgene plasmid # 26034 ; http://n2t.net/addgene:26034 ; RRID:Addgene_26034)

  • For your References section:

    MicroRNA-mediated integration of haemodynamics and Vegf signalling during angiogenesis. Nicoli S, Standley C, Walker P, Hurlstone A, Fogarty KE, Lawson ND. Nature. 2010 Apr 22. 464(7292):1196-200. 10.1038/nature08889 PubMed 20364122
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