pGEXtev-HMG54kd
(Plasmid #26030)

• Depositing Lab: Johann Deisenhofer
• Publication: Istvan et al EMBO J. 2000 Mar 1. 19(5):819-30.

Backbone

• Vector backbone: pGEX-cs
• Backbone size w/o insert (bp): 5043
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: 3-hydroxy-3-methylglutaryl-CoA reductase
• Alt name: HMG-CoA reductase, isoform 1
• Alt name: HMGCR
• Species: H. sapiens (human)
• Insert Size (bp): 1389
• Mutation: Catalytic domain (amino acids 426-488) Methionine 485 to Isoleucine mutation.
• Entrez Gene: HMGCR (a.k.a. LDLCQ3, LGMDR28, MYPLG)
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NCO1 (unknown if destroyed)
• 3′ cloning site: EcoR1 (unknown if destroyed)
• 5′ sequencing primer: pGEX-5
• 3′ sequencing primer: pGEX-3

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry