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Addgene

458 p5E-uasE1b
(Plasmid #26026)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26026 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pENTR
  • Backbone size w/o insert (bp) 2605
  • Vector type
    Entry vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    E1B basal promoter + UAS repeats
  • Insert Size (bp)
    522

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site attL1 (not destroyed)
  • 3′ cloning site attR2 (not destroyed)
  • 5′ sequencing primer M13pUC-fwd
  • 3′ sequencing primer M13rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

"p5E" refers to 5' entry clones flanked by L4-R1.

There are 2 mismatches between depositor's sequence and Addgene's quality control sequence. These mismatches do not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    458 p5E-uasE1b was a gift from Nathan Lawson (Addgene plasmid # 26026 ; http://n2t.net/addgene:26026 ; RRID:Addgene_26026)
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