pMXs-Ms-c-Myc-W136E
(Plasmid #26024)

• Depositing Lab: Shinya Yamanaka
• Publication: Nakagawa et al Proc Natl Acad Sci U S A. 2010 Jul 26. ():.

Backbone

• Vector backbone: pMXs
• Backbone manufacturer: Dr. Toshio Kitamura of the University of Tokyo
• Backbone size w/o insert (bp): 6340
• Vector type: Mammalian Expression, Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: c-Myc
• Alt name: Myc2; Nird; Niard; Myc
• Species: M. musculus (mouse)
• Insert Size (bp): 1320
• Mutation: W136E
• Entrez Gene: Myc (a.k.a. Myc2, Niard, Nird, bHLHe39)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ cloning site: attR1 (not destroyed)
• 3′ cloning site: attR2 (not destroyed)
• 5′ sequencing primer: pMX-S1811 (GAC GGC ATC GCA GCT TGG ATA CAC)
• 3′ sequencing primer: pMX-TgAS (GAC ATG GCC TGC CCG GTT ATT ATT)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pMXs is from Dr. Toshio Kitamura of the University of Tokyo, the Institute of Medical Science. If you use this plasmid in a paper, please cite: Retrovirus-mediated gene transfer and expression cloning: powerful tools in functional genomics. Exp Hematol. 2003 Nov;31(11):1007-14. Kitamura T, Koshino Y, Shibata F, Oki T, Nakajima H, Nosaka T, Kumagai H. Mutation(s) made by Provider lab: W136E

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry