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Addgene

VinculinTS
(Plasmid #26019)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26019 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5428
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Vinculin tension sensor
  • Alt name
    VinTS
  • Insert Size (bp)
    4764

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HINDIII (not destroyed)
  • 3′ cloning site EcoR1 (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note that this vector lacks the SP6 sequence present in pcDNA3.1. There is a small insertion in the vector sequence, upstream of Vinculin. Plasmid function should not be affected.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    VinculinTS was a gift from Martin Schwartz (Addgene plasmid # 26019 ; http://n2t.net/addgene:26019 ; RRID:Addgene_26019)

  • For your References section:

    Measuring mechanical tension across vinculin reveals regulation of focal adhesion dynamics. Grashoff C, Hoffman BD, Brenner MD, Zhou R, Parsons M, Yang MT, McLean MA, Sligar SG, Chen CS, Ha T, Schwartz MA. Nature. 2010 Jul 8. 466(7303):263-6. 10.1038/nature09198 PubMed 20613844
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