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Addgene

pSVK3-Flag-MAN1
(Plasmid #26002)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26002 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSVK3
  • Backbone size w/o insert (bp) 3919
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LEMD3 LEM domain containing 3
  • Alt name
    MAN1
  • Alt name
    LEMD3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2733
  • GenBank ID
    NM_001167614
  • Entrez Gene
    LEMD3 (a.k.a. MAN1)
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (destroyed during cloning)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer SV40pro-F
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSVK3-Flag-MAN1 was a gift from Howard Worman (Addgene plasmid # 26002 ; http://n2t.net/addgene:26002 ; RRID:Addgene_26002)

  • For your References section:

    Intracellular trafficking of MAN1, an integral protein of the nuclear envelope inner membrane. Wu W, Lin F, Worman HJ. J Cell Sci. 2002 Apr 1. 115(Pt 7):1361-71. PubMed 11896184
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