MSCV T274V S392A KSR1 IRES GFP
(Plasmid #25979)

• Depositing Lab: Rob Lewis
• Publication: Razidlo et al J Biol Chem. 2004 Nov 12. 279(46):47808-14.

Backbone

• Vector backbone: MSCV
• Backbone size w/o insert (bp): 6500
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: GFP

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Kinase suppressor of Ras 1
• Alt name: KSR1
• Species: M. musculus (mouse)
• Insert Size (bp): 2700
• Mutation: changed threonine 274 to valine and serine 392 to alanine
• GenBank ID: NM_013571
• Entrez Gene: Ksr1 (a.k.a. B-KSR1, D11Bhm183e, D11Bhm184e, Ksr, RSU2, hb, mKSR1)
• Tag / Fusion Protein:
  • Flag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoR1 (not destroyed)
• 3′ cloning site: Sal1/Xho1 (destroyed during cloning)
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Resource Information

• A portion of this plasmid was derived from a plasmid made by: M. Therrien

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry