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MSCV T274V KSR1 IRES GFP
(Plasmid #25977)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 25977 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    MSCV
  • Backbone size w/o insert (bp) 6500
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    GFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Kinase suppressor of Ras 1
  • Alt name
    KSR1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2700
  • Mutation
    changed threonine 274 to valine
  • GenBank ID
    NM_013571
  • Entrez Gene
    Ksr1 (a.k.a. B-KSR1, D11Bhm183e, D11Bhm184e, Ksr, RSU2, hb, mKSR1)
  • Tag / Fusion Protein
    • Flag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site Sal1/Xho1 (destroyed during cloning)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    M. Therrien

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MSCV T274V KSR1 IRES GFP was a gift from Rob Lewis (Addgene plasmid # 25977 ; http://n2t.net/addgene:25977 ; RRID:Addgene_25977)

  • For your References section:

    Phosphorylation regulates KSR1 stability, ERK activation, and cell proliferation. Razidlo GL, Kortum RL, Haferbier JL, Lewis RE. J Biol Chem. 2004 Nov 12. 279(46):47808-14. 10.1074/jbc.M406395200 PubMed 15371409
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