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Addgene

pCMV5 N539
(Plasmid #25972)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 25972 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV5
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Kinase suppressor of Ras 1
  • Alt name
    KSR1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2700
  • Mutation
    Deleted amino acids 540-873
  • GenBank ID
    NM_013571
  • Entrez Gene
    Ksr1 (a.k.a. B-KSR1, D11Bhm183e, D11Bhm184e, Ksr, RSU2, hb, mKSR1)
  • Tag / Fusion Protein
    • Flag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site Xba1 (methylated) (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer hGH-PA-R
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    M. Therrien

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV5 N539 was a gift from Rob Lewis (Addgene plasmid # 25972 ; http://n2t.net/addgene:25972 ; RRID:Addgene_25972)

  • For your References section:

    Kinase suppressor of Ras inhibits the activation of extracellular ligand-regulated (ERK) mitogen-activated protein (MAP) kinase by growth factors, activated Ras, and Ras effectors. Joneson T, Fulton JA, Volle DJ, Chaika OV, Bar-Sagi D, Lewis RE. J Biol Chem. 1998 Mar 27. 273(13):7743-8. 10.1074/jbc.273.13.7743 PubMed 9516483
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