pCMV5 WT KSR1
(Plasmid #25970)

• Depositing Lab: Rob Lewis
• Publication: Joneson et al J Biol Chem. 1998 Mar 27. 273(13):7743-8.

Backbone

• Vector backbone: pCMV5
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Kinase suppressor of Ras 1
• Alt name: KSR1
• Species: M. musculus (mouse)
• Insert Size (bp): 2700
• GenBank ID: NM_013571
• Entrez Gene: Ksr1 (a.k.a. B-KSR1, D11Bhm183e, D11Bhm184e, Ksr, RSU2, hb, mKSR1)
• Tag / Fusion Protein:
  • Flag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoR1 (not destroyed)
• 3′ cloning site: Kpn1 (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: hGH-PA-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: M. Therrien
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry