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pCDNA3 14 mut B-Myb
(Plasmid #25967)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 25967 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Bacterial Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    B-Myb
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2650
  • Mutation
    Changed threonines 267, 408, 443, 447 490, 497, 519, 522 and 524 to valines and serines 283, 343, 396, 424 and 581 to alanines
  • GenBank ID
    X70472
  • Entrez Gene
    Mybl2 (a.k.a. B-Myb, Bmyb)
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site Xba1 (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    R.J. Watson Ludwig Institute for Cancer Research, St Mary's Hospital Medical School, London, UK

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNA3 14 mut B-Myb was a gift from Rob Lewis (Addgene plasmid # 25967 ; http://n2t.net/addgene:25967 ; RRID:Addgene_25967)

  • For your References section:

    Effects of B-Myb on gene transcription: phosphorylation-dependent activity ans acetylation by p300. Johnson LR, Johnson TK, Desler M, Luster TA, Nowling T, Lewis RE, Rizzino A. J Biol Chem. 2002 Feb 8. 277(6):4088-97. 10.1074/jbc.M105112200 PubMed 11733503
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