pCDNA3 WT B-Myb
(Plasmid #25965)

• Depositing Lab: Rob Lewis
• Publication: Johnson et al J Biol Chem. 1999 Dec 17. 274(51):36741-9.

Backbone

• Vector backbone: pCDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: B-Myb
• Species: H. sapiens (human)
• GenBank ID:
• Entrez Gene: MYBL2 (a.k.a. B-MYB, BMYB)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoR1 (not destroyed)
• 3′ cloning site: Xba1 (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• A portion of this plasmid was derived from a plasmid made by: R.J. Watson Ludwig Institute for Cancer Research, St Mary's Hospital Medical School, London, UK
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry