cp193g7
(Plasmid
#25950)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 25950 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBad/HisB
- Backbone size w/o insert (bp) 4100
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namecp193g7
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Alt namecp193
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SpeciesD. Striata
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Insert Size (bp)700
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Tag
/ Fusion Protein
- His (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (unknown if destroyed)
- 3′ cloning site HindIII (unknown if destroyed)
- 5′ sequencing primer RECA1 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
cp193g7 was a gift from Robert Campbell (Addgene plasmid # 25950 ; http://n2t.net/addgene:25950 ; RRID:Addgene_25950) -
For your References section:
Circularly permuted monomeric red fluorescent proteins with new termini in the beta-sheet. Carlson HJ, Cotton DW, Campbell RE. Protein Sci. 2010 Jun 2. ():. 10.1002/pro.428 PubMed 20521333