pcDNA3-PalmPalm-ClopHensor
(Plasmid #25940)

• Depositing Lab: Daniele Arosio
• Publication: Arosio et al Nat Methods. 2010 Jul . 7(7):516-8.

Backbone

• Vector backbone: pcDNA3.1(+)
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5344
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PalmPalm-ClopHensor
• Alt name: E2GFP fused to DsRed-monomer
• Species: Aq. Victoria and Discosoma sp
• Insert Size (bp): 1542
• Mutation: N-term (20AA) of Gap-43
• Tags / Fusion Proteins:
  • Gap20 (N terminal on insert)
  • Strep-II (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (unknown if destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest for 25940
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry