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HA-HIF2α P405A/N847A Δ450-572-pBabe-Puro
(Plasmid #25936)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 25936 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    HA-pBabe-puro
  • Backbone size w/o insert (bp) 5140
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Hypoxia inducible factor 2 alpha
  • Alt name
    HIF2a
  • Alt name
    EPAS1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2286
  • Mutation
    cDNA changed amino acids: Proline 405 to Alanine Asparagine 847 to Alanine internal deletion of Amino acids 450 - 527 (NTAD = N-terminal transactivation domain) This mutant is called "M4" in Yan et al. 2007, Mol Cell Biol, 27, 209-2-102 .
  • GenBank ID
    HSU81984
  • Entrez Gene
    EPAS1 (a.k.a. ECYT4, HIF2A, HLF, MOP2, PASD2, bHLHe73)
  • Tag / Fusion Protein
    • HA-tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH I (not destroyed)
  • 3′ cloning site EcoR I (destroyed during cloning)
  • 5′ sequencing primer pBabe-5'
  • 3′ sequencing primer pBabe-3'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HA-HIF2α P405A/N847A Δ450-572-pBabe-Puro was a gift from William Kaelin (Addgene plasmid # 25936 ; http://n2t.net/addgene:25936 ; RRID:Addgene_25936)

  • For your References section:

    The hypoxia-inducible factor 2alpha N-terminal and C-terminal transactivation domains cooperate to promote renal tumorigenesis in vivo. Yan Q, Bartz S, Mao M, Li L, Kaelin WG. Mol Cell Biol. 2007 Mar . 27(6):2092-102. 10.1128/MCB.01514-06 PubMed 17220275
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