pEGFP-RapR-FAK-YM-Y397F
(Plasmid #25930)

• Depositing Lab: Klaus Hahn
• Publication: Karginov et al Nat Biotechnol. 2010 Jun 27. ():.

Backbone

• Vector backbone: pEGFP-C1
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: RapR-FAK
• Species: H. sapiens (human), M. musculus (mouse)
• Insert Size (bp): 3400
• Mutation: This pEGFP-RapR-FAK-YM-Y397F plasmid contains the FAK mutations Y180A and M183A, as well as the mutation Y397F. (The uploaded sequence is based on wt). FAK is murine and the FKBP fragment (called RapR in the plasmid name) is human.
• Entrez Gene: Ptk2 (a.k.a. FADK 1, FAK, FRNK, Fadk, p125FAK)
• Tag / Fusion Protein:
  • EGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV-Fw

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry