pEGFP-RapR-FAK-YM
(Plasmid #25928)

• Depositing Lab: Klaus Hahn
• Publication: Karginov et al Nat Biotechnol. 2010 Jun 27. ():.

Backbone

• Vector backbone: pEGFP-C1
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: RapR-FAK
• Species: H. sapiens (human), M. musculus (mouse)
• Insert Size (bp): 3400
• Mutation: This pEGFP-RapR-FAK-YM plasmid contains the FAK mutations Y180A and M183A. (The uploaded sequence is based on wt). FAK is murine and the FKBP fragment (called RapR in the plasmid name) is human.
• Entrez Gene: Ptk2 (a.k.a. FADK 1, FAK, FRNK, Fadk, p125FAK)
• Tag / Fusion Protein:
  • EGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV-Fw

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry