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Addgene

pMAD-Gate1
(Plasmid #25916)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 25916 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pACT
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 5566
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Growth instructions
    Strain (ccdB Survival Strain): F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 araΔ139 Δ(ara-leu)7697 galU galK rpsL (StrR) endA1 nupG fhuA::IS2 ; Growth condition: 37°C
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    None
  • Insert Size (bp)
    1711
  • Tag / Fusion Protein
    • VP16 Fusion Protein (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRV (not destroyed)
  • 3′ cloning site EcoRV (not destroyed)
  • 5′ sequencing primer ATTCCCGGGGATCTCGAC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMAD-Gate1 was a gift from Kamil Onder (Addgene plasmid # 25916 ; http://n2t.net/addgene:25916 ; RRID:Addgene_25916)

  • For your References section:

    Coupled Yeast 2-Hybrid-Mammalian 2-Hybrid Reading-Frame-Independent and Site-Specific Recombinational Cloning Vector System. Maier CJ, Maier RH, Hintner H, Bauer JW, Onder K. Assay Drug Dev Technol. 2010 Jun 1. ():. 10.1089/adt.2009.0266 PubMed 20515414
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