Skip to main content
Addgene

phtsResA
(Plasmid #25914)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 25914 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pET21a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5443
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    His-tagged soluble ResA
  • Alt name
    ResA
  • Species
    Bacillus subtilis
  • Insert Size (bp)
    426
  • Mutation
    Start at Serine 37 to remove n-terminal transmembrane helix anchor.
  • GenBank ID
    P35160
  • Entrez Gene
    resA
  • Tag / Fusion Protein
    • His (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    phtsResA was a gift from Johann Deisenhofer (Addgene plasmid # 25914 ; http://n2t.net/addgene:25914 ; RRID:Addgene_25914)

  • For your References section:

    Mechanism of substrate specificity in Bacillus subtilis ResA, a thioredoxin-like protein involved in cytochrome c maturation. Colbert CL, Wu Q, Erbel PJ, Gardner KH, Deisenhofer J. Proc Natl Acad Sci U S A. 2006 Mar 21. 103(12):4410-5. 10.1073/pnas.0600552103 PubMed 16537372
Related items: