PJ14
(Plasmid
#25912)
-
Depositing Lab
-
Publication
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 25912 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepBR322
-
Backbone manufacturerATCC
- Backbone size w/o insert (bp) 4361
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameIg germline mu switch region
-
SpeciesM. musculus (mouse)
-
Insert Size (bp)5000
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (unknown if destroyed)
- 3′ cloning site BamHI (unknown if destroyed)
- 5′ sequencing primer n/a
- 3′ sequencing primer pBRrevBam_primer (Common Sequencing Primers)
Resource Information
-
Article Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Includes a 5 kb EcoRI/BamHI fragment with Smu (3 kb deletion relative to the germline) and both 5' and 3' flanking sequences.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
PJ14 was a gift from Kenneth Marcu (Addgene plasmid # 25912 ; http://n2t.net/addgene:25912 ; RRID:Addgene_25912) -
For your References section:
On immunoglobulin heavy chain gene switching: two gamma 2b genes are rearranged via switch sequences in MPC-11 cells but only one is expressed. Lang RB, Stanton LW, Marcu KB. Nucleic Acids Res. 1982 Jan 22. 10(2):611-30. 10.1093/nar/10.2.611 PubMed 6278424