p64-101
(Plasmid
#25911)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 25911 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBR322
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Backbone manufacturerATCC
- Backbone size w/o insert (bp) 4361
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameIg germline gamma-1 switch region alpha
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SpeciesM. musculus (mouse)
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Insert Size (bp)5000
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (unknown if destroyed)
- 3′ cloning site HindIII (unknown if destroyed)
- 5′ sequencing primer n/a
- 3′ sequencing primer pBRrevBam (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Includes a 5 kb fragment germline, which includes most of the Calpha switch region (Salpha) and part of Calpha.
NB: CH switch (S) regions
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p64-101 was a gift from Kenneth Marcu (Addgene plasmid # 25911 ; http://n2t.net/addgene:25911 ; RRID:Addgene_25911) -
For your References section:
Nucleotide sequence and properties of the murine gamma 3 immunoglobulin heavy chain gene switch region: implications for successive C gamma gene switching. Stanton LW, Marcu KB. Nucleic Acids Res. 1982 Oct 11. 10(19):5993-6006. 10.1093/nar/10.19.5993 PubMed 6292864