TRPM4
(Plasmid #25903)

• Depositing Lab: Craig Montell
• Publication: Xu et al Proc Natl Acad Sci U S A. 2001 Sep 11. 98(19):10692-7.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5428
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: TRPM4
• Species: H. sapiens (human)
• Insert Size (bp): 1040
• Entrez Gene: TRPM4 (a.k.a. LTrpC4, PFHB1B, TRPM4B, hTRPM4)
• Promoter: CMV
• Tag / Fusion Protein:
  • FLAG (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: XbaI (unknown if destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The expressed sequence tag clone H18835, encoding a fragment of TRPM4, was used to screen human brain, placenta, and testis cDNA libraries. Several positive clones were recovered, including a 4.0-kb cDNA encoding a predicted protein of 1,040 aa. The TRPM4 cDNA was subcloned into the vector pcDNA3 (Invitrogen) to create pTRPM4. The coding region was fused to an N-terminal FLAG tag.