pw35Gal4
(Plasmid
#25901)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 25901 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepW35
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Backbone manufacturerKent Golic
- Backbone size w/o insert (bp) 8842
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameGAL4
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)3150
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Entrez GeneGAL4 (a.k.a. YPL248C, GAL81)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer needs custom primer (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
To facilitate the insertion of the GAL4 gene in a given Drosophila gene by
homologous recombination, we created the pw35GAL4 vector by subcloning the yeast
GAL4 gene into the pw35 vector
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pw35Gal4 was a gift from Craig Montell (Addgene plasmid # 25901 ; http://n2t.net/addgene:25901 ; RRID:Addgene_25901) -
For your References section:
A Drosophila gustatory receptor essential for aversive taste and inhibiting male-to-male courtship. Moon SJ, Lee Y, Jiao Y, Montell C. Curr Biol. 2009 Oct 13. 19(19):1623-7. 10.1016/j.cub.2009.07.061 PubMed 19765987
Map uploaded by the depositor.
