pNANOG-Luc
(Plasmid #25900)

• Depositing Lab: Ren-he Xu
• Publication: Xu et al Cell Stem Cell. 2008 Aug 7. 3(2):196-206.

Backbone

• Vector backbone: pGL4.10
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 4242
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: NANOG promoter
• Species: H. sapiens (human)
• Insert Size (bp): 404
• Mutation: None
• GenBank ID: NT_086792
• Entrez Gene: NANOG
• Tag / Fusion Protein:
  • Luciferase (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Xho I (not destroyed)
• 3′ cloning site: Bgl II (not destroyed)
• 5′ sequencing primer: RVprimer3
• 3′ sequencing primer: Ref to Promega

Resource Information

• Articles Citing this Plasmid:
  • 8 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry