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Addgene

(P79) OCN/Wnt 10b transgene
(Plasmid #25878)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 25878 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCRII
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4000
  • Vector type
    Mammalian Expression, Mouse Targeting

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Wnt10b
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Wnt10b (a.k.a. Wnt1, Wnt12)
  • Tags / Fusion Proteins
    • OCN promoter (N terminal on insert)
    • B-globin intron/poly A coding sequence (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site SalI (destroyed during cloning)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See attached map for details.

The entire OCN-Wnt10b-b-globin product can be excised with HindIII/XhoI for a ~6.1 kB fragment

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    (P79) OCN/Wnt 10b transgene was a gift from Ormond MacDougald (Addgene plasmid # 25878 ; http://n2t.net/addgene:25878 ; RRID:Addgene_25878)
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