(G38) pCRII-Wnt10b/b-globin
(Plasmid
#25877)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 25877 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCRII
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 4000
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameWnt10b / b-globin
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SpeciesM. musculus (mouse)
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Insert Size (bp)2200
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Entrez GeneWnt10b (a.k.a. Wnt1, Wnt12)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SpeI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer SP6
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
(G38) pCRII-Wnt10b/b-globin was a gift from Ormond MacDougald (Addgene plasmid # 25877 ; http://n2t.net/addgene:25877 ; RRID:Addgene_25877) -
For your References section:
Wnt10b inhibits development of white and brown adipose tissues. Longo KA, Wright WS, Kang S, Gerin I, Chiang SH, Lucas PC, Opp MR, MacDougald OA. J Biol Chem. 2004 Aug 20. 279(34):35503-9. 10.1074/jbc.M402937200 PubMed 15190075
Map uploaded by the depositor.
![](https://media.addgene.org/data/easy-thumbnails/data/75/51/568a2000-af63-11e0-90fe-003048dd6500.jpeg.940x940_q85_autocrop.png)