Skip to main content
Addgene

(G38) pCRII-Wnt10b/b-globin
(Plasmid #25877)

Print

Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 25877 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pCRII
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4000
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Wnt10b / b-globin
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2200
  • Entrez Gene
    Wnt10b (a.k.a. Wnt1, Wnt12)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SpeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    (G38) pCRII-Wnt10b/b-globin was a gift from Ormond MacDougald (Addgene plasmid # 25877 ; http://n2t.net/addgene:25877 ; RRID:Addgene_25877)

  • For your References section:

    Wnt10b inhibits development of white and brown adipose tissues. Longo KA, Wright WS, Kang S, Gerin I, Chiang SH, Lucas PC, Opp MR, MacDougald OA. J Biol Chem. 2004 Aug 20. 279(34):35503-9. 10.1074/jbc.M402937200 PubMed 15190075
Related items:
Commonly requested with: