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Addgene

pcDNA3.1 Hygro EYFP H148Q/I152L
(Plasmid #25874)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 25874 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1 Hygro
  • Backbone size w/o insert (bp) 5600
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EYFP H148Q/I152L
  • Species
    A. victoria
  • Insert Size (bp)
    700
  • Mutation
    EYFP from pEYFP (Clontech) with H148Q and I152L mutations

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site bamHI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGHrev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1 Hygro EYFP H148Q/I152L was a gift from Peter Haggie (Addgene plasmid # 25874 ; http://n2t.net/addgene:25874 ; RRID:Addgene_25874)

  • For your References section:

    Green fluorescent protein-based halide indicators with improved chloride and iodide affinities. Galietta LJ, Haggie PM, Verkman AS. FEBS Lett. 2001 Jun 22. 499(3):220-4. 10.1016/S0014-5793(01)02561-3 PubMed 11423120
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