qz5
(Plasmid #25867)

• Depositing Lab: Bruce Conklin
• Publication: Conklin et al Nature. 1993 May 20. 363(6426):274-6.

Backbone

• Vector backbone: pcDNA1
• Backbone size w/o insert (bp): 4000
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin and Tetracycline, 100 & 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10/P3
• Growth instructions: Plasmid contains SupF. Grow in TOP10/P3 cells.
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Gq alpha
• Alt name: G protein alpha subunit
• Alt name: Gnaq
• Species: M. musculus (mouse)
• Mutation: Replacement of the five carboxyl-terminal amino acids of Gq alpha (EYNLV) with those of Gz alpha(YIGLC)
• Entrez Gene: Gnaq (a.k.a. 1110005L02Rik, 6230401I02Rik, Dsk1, Dsk10, Galphaq, Gq, GqI)
• Tag / Fusion Protein:
  • HA (see comments)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: NsiI (unknown if destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SP6

Resource Information

• Supplemental Documents:
  • G-protein chimera user manual

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This is Gq alpha with the C-terminal amino acids changed from Gq alpha to Gz alpha residues (EYNLV to YIGLC). Works the same as qi5 and is the least popular since no one knows what Gz alpha really does in nature. Since qz5 is not sensitive to pertussis toxin, qz5 may be the only G protein activated by a Gi-coupled receptor in cells treated with pertussis toxin. This trick can be experimentally useful in settings where you want experimental control of the exact G protein and the receptor that is activated. It is theoretically possible that this construct will work better than the other constructs for particular receptors, but the Conklin lab has not seen this happen yet.

Contains an internal hemaglutinin epitope tag (DVPDYA) that has no effect on receptor coupling.

Please see supplemental document for G-protein chimera user manual.