Monosiga cDNA library
(Pooled Library #25864)

• Purpose:

  cDNA library for Monosiga brevicollis.

• Vector Backbone:

  pSport 1

• Depositing Labs: Sean Carroll, Nicole King
• Publication: King et al Proc Natl Acad Sci U S A. 2001 Dec 18. 98(26):15032-7.

Library Details

• Species: Monosiga brevicollis

Library Shipping

Each library is delivered in microcentrifuge tubes on blue ice. A tube's contents will not necessarily be frozen. For best results, minimize freeze/thaw cycles.

• Volume: ∼100 µL
• Concentration: 100 ng/µL

Resource Information

• Addgene Data:
  • Quality control sequencing of Monosiga cDNA library

Terms and Licenses

Academic/Nonprofit Terms:

• UBMTA

Industry Terms:

• Not Available to Industry

Trademarks:

• Zeocin® is an InvivoGen trademark.

Depositor Comments

For construction of this cDNA library, double-stranded cDNA was synthesized with SuperScriptII RT and DNA polymerase I and ligated to adapters containing a SalI restriction site (GIBCO/BRL). To remove residual adapters and enrich for full-length fragments, cDNA was first size-fractionated by column chromatography and then ligated to predigested pSportI vector (GIBCO/BRL). Transformation of DH5α with the ligated DNA yielded a plasmid library with approximately 8.8 x 10⁴ colony-forming units.

Note: Addgene has sequenced part of this library. See the Addgene Data link above for more information.