pZE31ms2
(Plasmid #25860)

• Depositing Lab: Philippe Cluzel
• Publication: Le et al Proc Natl Acad Sci U S A. 2005 Jun 28. 102(26):9160-4.

Backbone

• Vector backbone: pZE31
• Backbone manufacturer: Lutz and Bujard, Nucleic Acids Research 25 (1997) 1203-1210.
• Backbone size w/o insert (bp): 2000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ms2 binding site (x2)
• Insert Size (bp): 150
• Mutation: none

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: PLtetO-1

Resource Information

• A portion of this plasmid was derived from a plasmid made by: ms2 binding sequence was amplified from pIIIMS2-2 plasmid from K Bloom UNC Chapel Hill. Vector provided to C. Guet from H. Bujard.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry