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pMX-Brg1
(Plasmid #25855)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 25855 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMX
  • Backbone manufacturer
    Toshio Kitamura
  • Backbone size w/o insert (bp) 4628
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Brahma related gene 1
  • Alt name
    Smarca4
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    5521
  • GenBank ID
    BC079560
  • Entrez Gene
    Smarca4 (a.k.a. BAF190A, Brg1, HP1-BP72, SNF2beta, SW1/SNF, b2b508.1Clo, b2b692Clo)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (unknown if destroyed)
  • 3′ cloning site Not1 (unknown if destroyed)
  • 5′ sequencing primer pBMN 5'
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMX-Brg1 was a gift from Hans Schöler (Addgene plasmid # 25855 ; http://n2t.net/addgene:25855 ; RRID:Addgene_25855)

  • For your References section:

    Chromatin-Remodeling Components of the BAF Complex Facilitate Reprogramming. Singhal N, Graumann J, Wu G, Arauzo-Bravo MJ, Han DW, Greber B, Gentile L, Mann M, Schoeler HR. Cell. 2010 Jun 11. 141(6):943-55. 10.1016/j.cell.2010.04.037 PubMed 20550931
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