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pGL3-DICER-Prom Ebox double mut
(Plasmid #25854)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 25854 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL3 basic
  • Backbone size w/o insert (bp) 5394
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DICER-Promoter Ebox double mut
  • Species
    H. sapiens (human); gene ID 23405
  • Insert Size (bp)
    2542
  • Entrez Gene
    DICER1 (a.k.a. DCR1, Dicer, Dicer1e, GLOW, HERNA, K12H4.8-LIKE, MNG1, RMSE2, aviD)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer RVprimer3
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL3-DICER-Prom Ebox double mut was a gift from David E. Fisher (Addgene plasmid # 25854 ; http://n2t.net/addgene:25854 ; RRID:Addgene_25854)

  • For your References section:

    Lineage-specific transcriptional regulation of DICER by MITF in melanocytes. Levy C, Khaled M, Robinson KC, Veguilla RA, Chen PH, Yokoyama S, Makino E, Lu J, Larue L, Beermann F, Chin L, Bosenberg M, Song JS, Fisher DE. Cell. 2010 Jun 11. 141(6):994-1005. 10.1016/j.cell.2010.05.004 PubMed 20550935
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